Based on multi-omics combined analysis, efficient gene editing technology and DBTL cycle optimization, the rapid identification, assembly and precise regulation of complex metabolic pathways can be realized, and the biosynthetic routes of target products can be assembled into the metabolic pathways of chassis cells. Biomacromolecules with specific catalytic regulation functions are transformed into standardized components, and new modules and routes are constructed to build a new generation of artificial intelligence cell factories.